The non-invasive quantification of advanced glycation end products (AGEs) in skin tissue holds significant promise for the early screening and monitoring of diabetes and other age-related chronic diseases. However, this endeavor is fundamentally challenged by the severe spectral overlap among AGEs and other dominant endogenous fluorophores, including reduced nicotinamide adenine dinucleotide (NADH), flavin adenine dinucleotide (FAD), and collagen (COL). Conventional detection strategies, such as single-wavelength excitation with full-spectrum integration, are highly susceptible to cross-interference from these coexisting components, leading to substantial inaccuracies. To overcome this critical bottleneck, this study aims to establish a robust methodological framework for high-specificity quantification of AGEs by systematically evaluating state-of-the-art spectral unmixing algorithms and developing a principled strategy for selecting an optimal set of discrete excitation wavelengths within a well-controlled simulated system.
A comprehensive dataset is constructed, comprising both simulated and experimentally measured excitation-emission matrix (EEM) spectra of the four-component mixture (AGEs, NADH, FAD, and COL). The simulated data is generated under four distinct noise levels (0.00, 0.05, 0.10, 0.20) to mimic varying signal-to-noise ratios, while the experimental data is acquired from prepared aqueous solutions. Three prominent unmixing algorithms—non-negative matrix factorization (NMF), parallel factor analysis (PARAFAC), and Richardson-Lucy spectral unmixing (RLSU)—are rigorously benchmarked against the ground-truth concentrations. Their performance is evaluated using the coefficient of determination (
The algorithmic benchmark clearly identified PARAFAC as the superior performer, consistently achieving the highest
This work successfully demonstrates that the PARAFAC algorithm, coupled with a rationally selected set of discrete excitation wavelengths, provides a powerful and robust solution for the spectral unmixing of the four key skin autofluorescent biomarkers in a simulated environment. The identified wavelength combination {345, 370, 385, 420, 435, 455} nm offers a concrete blueprint for the future development of compact, low-cost, and high-specificity optical devices for non-invasive skin AGEs detection. It is crucial to note that this study is based on an idealized "solution model" that does not account for the light scattering and absorption effects present in real biological tissue. Future research will focus on validating this methodological framework in more realistic scattering phantoms and ultimately in in vivo human trials to pave the way for clinical translation.
Underwater wireless optical communication (UWOC) is recognized as a promising technology for high-speed underwater data transmission due to its high bandwidth, low latency, and immunity to electromagnetic interference. However, the UWOC channel is inherently complex, especially in multipath environments, which induces severe frequency-selective fading, leading to inter-symbol interference (ISI) and inter-carrier interference (ICI). These impairments degrade the accuracy of channel estimation, which is crucial for reliable signal recovery at the receiver. Traditional estimation methods, such as least squares (LS) and linear minimum mean square error (LMMSE), exhibit limitations in strong multipath conditions: LS is highly sensitive to noise, while LMMSE relies heavily on accurate channel statistical information, which is difficult to obtain in dynamic underwater environments. Although deep learning approaches, such as deep neural networks (DNN) and gated recurrent units (GRU), are introduced to improve estimation, they often fail to fully exploit the frequency-domain correlations among subcarriers or adaptively focus on key features. Therefore, there is a pressing need for a robust and efficient channel estimation method that can maintain high accuracy under limited pilot overhead and without the reliance on a cyclic prefix (CP), thereby enhancing the spectral efficiency of UWOC systems.
This paper proposes a novel neural network-based channel estimator named BSD (BiLSTM-Self-Attention-DNN Network) for DC-biased optical orthogonal frequency division multiplexing (DCO-OFDM) systems in UWOC. The BSD network is designed to perform end-to-end mapping from received signals to transmitted symbols, bypassing explicit channel estimation and equalization. The network architecture comprises three main components: a bidirectional long short-term memory (BiLSTM) layer, a self-attention (SA) layer, and a dense neural network (DNN) layer. The BiLSTM layer processes the subcarrier sequence in both forward and backward directions, capturing long-range dependencies and frequency-domain smoothness characteristic of multipath channels. The SA layer then computes attention weights across subcarriers, adaptively emphasizing those with critical information (e.g., near pilot locations or less affected by fading) while suppressing noisy ones. Finally, the DNN layer performs nonlinear transformation and dimensionality reduction to recover the transmitted bit stream. To train the BSD network, a comprehensive dataset is generated using Monte Carlo simulations that emulate realistic underwater optical channels. The simulation models photon propagation in two typical water types: clear ocean and coastal ocean, incorporating effects such as absorption, scattering, and boundary reflections. Key parameters include a wavelength of 470 nm, water depth of 15 m, and anisotropic factor of 0.8. The DCO-OFDM system is configured with 192 subcarriers, 16-QAM modulation, and variable pilot numbers (8 to 64). The network is trained offline using the mean squared error (MSE) loss function and the Adam optimizer under a signal-to-noise ratio (SNR) of 25 dB.
The performance of the BSD estimator is evaluated through extensive simulations and compared with traditional methods (LS, LMMSE) and neural network benchmarks (DNN, GRU). In both clear and coastal ocean channels with CP inserted and 64 pilots, BSD achieves the lowest bit error rate (BER) across various SNR levels. For instance, at 30 dB SNR in clear ocean, BSD attains a BER of
This paper presents a BSD neural network for channel estimation in underwater DCO-OFDM systems. By integrating BiLSTM for sequence dependency modeling, self-attention for adaptive feature weighting, and DNN for nonlinear regression, the proposed method effectively mitigates multipath-induced distortions. Simulation results validate that BSD outperforms existing techniques in terms of BER performance across various water types, pilot counts, and CP conditions. Crucially, BSD demonstrates strong robustness in scenarios with limited pilots and without CP, thereby reducing spectral overhead and enhancing system spectral efficiency. The use of Monte Carlo-simulated channel data ensures that the model is trained on realistic underwater conditions, improving its practical applicability. The BSD estimator offers a promising deep learning-based solution for high-performance and resource-efficient UWOC systems.
Low-pressure gas discharges exhibit broad application prospects in plasma display panels, medical sterilization, surface treatment of materials, and protection of vacuum electronic devices. However, their discharge processes are often characterized by strong randomness of discharge paths and poor triggering stability, which severely limit precise control and practical engineering applications under low-pressure conditions. Femtosecond laser pulses propagating in gases can generate plasma channels with finite electron density, providing a pre-ionized pathway for electrical breakdown and enabling spatial confinement and temporal triggering of the discharge process. Nevertheless, existing studies on femtosecond laser-triggered discharges have mainly focused on atmospheric-pressure environments, while systematic investigations into the guiding capability, spatiotemporal stability, and plasma lifetime under low-pressure conditions remain insufficient. Motivated by this gap, the present study systematically investigates femtosecond laser-triggered discharges in air over a wide pressure range from 1 to 100 kPa. Particular emphasis is placed on elucidating the effects of ambient pressure on discharge breakdown characteristics, spatial stability, and temporal stability. The objective is to reveal the coupling mechanisms between femtosecond laser-produced plasma and external electric fields under low-pressure conditions, thereby providing experimental insights for precise control of low-pressure discharges and the extension of their related applications.
A Ti∶sapphire femtosecond laser system is employed in the experiments, delivering pulses with a central wavelength of 800 nm, a pulse duration of 45 fs, a single-pulse energy of 3.5 mJ, and a repetition rate of 10 Hz. The laser beam is focused into a low-pressure chamber using a lens with a focal length of 300 mm to generate a filamentary plasma channel. A pair of tungsten electrodes with an interelectrode gap of 5 mm is installed inside the chamber and connected to a nanosecond-scale pulsed direct current (DC) high-voltage power supply. The ambient pressure inside the chamber is continuously adjustable in the range of 1 to 100 kPa. A digital delay generator is used to precisely control the time delay
In terms of spatial characteristics, free discharges exhibit noticeable path bending and nonuniform plasma distributions under both atmospheric and low-to-medium pressure conditions (middle column). In contrast, femtosecond laser-triggered discharges propagate strictly along the laser-produced plasma channel. At a pressure of approximately 10 kPa, a narrow discharge channel with a diameter of about 0.4 mm is formed, featuring a more concentrated and uniform radial emission profile (right column). Compared with the case at 50 kPa, the discharge channel diameter at 10 kPa is reduced to only 44%, indicating a significantly improved spatial resolution. When the pressure is further reduced to 1 kPa, both free discharges and laser-guided discharges degenerate into corona discharges due to insufficient electron density and collision frequency, preventing the formation of a stable breakdown channel. For pressures above 1 kPa, stable laser-guided discharges can be achieved, and the overall variation of the breakdown voltage threshold with pressure follows Paschen's law. In the pressure range of 2.5?10 kPa, the breakdown threshold varies slowly, whereas it increases approximately linearly with pressure at higher pressures. Compared with free discharges, the breakdown voltage threshold of femtosecond laser-triggered discharges is significantly reduced to 50.0%?66.7% of that of free discharges, with a more pronounced advantage observed on the rising branch of the Paschen curve. Regarding temporal stability, the low-pressure environment substantially improves the triggering characteristics of the discharge. At 10 kPa, the discharge delay time is significantly shortened, and the timing jitter remains below 5 ns over a wide range of applied voltages and delay times, reaching sub-nanosecond levels under certain conditions. Compared with atmospheric pressure, the effective triggering time window for femtosecond laser-triggered discharges at low pressure is greatly extended, reaching up to -400 μs under negative delay conditions, which is approximately 12 times wider than that at atmospheric pressure. Notably, for longer negative delay times ranging from -500 μs to -90 ms, although a complete discharge channel is not formed, fluorescence emission induced by residual plasma under the external electric field can still be observed. This phenomenon indicates that femtosecond laser pulses can generate low-density plasma remnants with lifetimes on the order of milliseconds in low-pressure gases. These plasma remnants can be re-excited by an external electric field, producing sustained fluorescence emission and exhibiting a visual effect analogous to "filament re-ignition".
This study systematically investigates the breakdown characteristics and spatiotemporal stability of femtosecond laser-triggered discharges under low-pressure conditions. The results demonstrate that, within an appropriate low-pressure range, femtosecond laser guidance can significantly reduce the breakdown voltage threshold, generate discharge channels with enhanced spatial resolution and superior temporal stability, and substantially broaden the effective triggering time window. Moreover, millisecond-lifetime residual plasma and its electric-field-induced re-excitation fluorescence are observed for the first time under low-pressure conditions. These findings provide experimental insights into the coupling mechanisms between laser-produced plasma and external electric fields at low pressure and offer a new technical pathway for precise control of low-pressure discharges and their applications in other fields.
Laser cladding technology, as a core means of surface strengthening and remanufacturing of key components in high-end equipment, has become essential for surface strengthening, damage repair, and functional gradient material preparation in high-end manufacturing industries such as aerospace, energy equipment, and medical devices. However, its transient thermal melting characteristics of"rapid heating and rapid cooling"can easily induce defects such as cracks, pores, and uneven microstructure in the cladding layer, seriously restricting the reliability and service life of the formed parts. Traditional process parameter optimization and powder characteristic adjustment encounter a significant"ceiling"effect, making it difficult to achieve deep defect suppression and performance improvement. In this context, external physical fields such as magnetic field, ultrasonic field, and thermal field are introduced to construct a closed-loop system of"energy field-assisted performance control in laser cladding". By actively intervening in the heat and mass transfer and solidification behavior of the melt pool, this approach provides an innovative path to break through technological bottlenecks and prepare high-quality cladding layers, holding great significance for promoting the engineering application of laser cladding technology and upgrading high-end equipment manufacturing.
The control mechanism of energy fields such as magnetic field, ultrasonic field, and thermal field on defect suppression (such as cracks, pores, and compositional segregation) and microstructure optimization (such as grain refinement) during laser cladding can be explained by"regulating the convective behavior of the melt pool"and"optimizing the solidification process". The magnetic field exerts a synergistic effect of"forced convection"through the thermoelectric magnetic effect, achieving grain refinement by disrupting columnar crystal growth through thermoelectric magnetic force and enhancing solute exchange in the melt pool through the thermoelectric electromagnetic effect. At the same time, the electromagnetic braking effect can effectively suppress splashing and natural convection of the melt pool, stabilize melt pool morphology and reduce defect formation. The ultrasonic field utilizes the cavitation effect and acoustic streaming effect to form a"macroscopic homogenization and microscopic nucleation strengthening" mechanism, breaking the growth mode of coarse columnar crystals under rapid solidification, promoting the transformation of columnar crystals into equiaxed crystals, achieving grain refinement and microstructure homogenization, and ultimately improving the comprehensive performance of the cladding layer such as hardness, wear resistance, and corrosion resistance. The thermal field focuses on temperature regulation, achieving the goals of regulating the temperature field of the melt pool, mitigating thermal stress accumulation, suppressing cracks and pore defects, and optimizing grain growth conditions through preheating or synchronous heating. However, a single energy field also has limitations such as a limited range of action and single performance optimization. The combination of multiple energy fields compensates for the limitations of a single energy field through the"thermal-mechanical" coupling effect, achieving coordinated defect suppression. In the ultrasonic-electromagnetic composite field, the Lorentz force expands and enhances the cavitation effect throughout the entire melt pool, maximizing undercooling of the melt pool and promoting the uniform distribution of the strengthening phase
The core of single energy field-assisted defect suppression and microstructure optimization lies in the reconstruction of solidification conditions, which creates conditions for grain refinement by actively intervening in the convective state of the melt pool and altering the ratio of temperature gradient to solidification rate. Multi-energy field combinations integrate the advantages of each field through the "thermal-mechanical"coupling effect, achieving synergistic suppression of multidimensional defects (cracks, pores, segregation) and yielding a performance improvement of
Submission Open:1 November 2026; Submission Deadline: 2 January 2027
Editor (s): Renmin Ma, Stephan Reitzenstein, Satoshi Iwamoto, Qing Gu, Juan Du
Submission Open:1 September 2026; Submission Deadline: 1 December 2026
Editor (s): Zhanghua Han, Maxim Gorkunov, Ivan Fernandez Corbaton
Special Issue on PROton BOron Nuclear fusion: from energy production to medical applicatiOns (2026)
Submission Open:14 July 2026; Submission Deadline: 28 April 2027